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Western blot was performed on nuclear extracts from HeLa cells (20 μg) with Med26 Polyclonal Antibody (bs-53124R), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk, followed by secondary antibody incubation.
Western blot was performed on nuclear extracts from HeLa cells (20 μg) with Med26 Polyclonal Antibody (bs-53124R), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk, followed by secondary antibody incubation.
Western blot was performed on nuclear extracts from HeLa cells (20 μg) with Med26 Polyclonal Antibody (bs-53124R), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk, followed by secondary antibody incubation.

Med26 Polyclonal Antibody

BS-53124R
Bioss
ApplicationsWestern Blot
Product group Antibodies
TargetMED26
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Overview

  • Supplier
    Bioss
  • Product Name
    Med26 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Applications Supplier
    WB(1:300-5000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    2 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID9441
  • Target name
    MED26
  • Target description
    mediator complex subunit 26
  • Target synonyms
    CRSP7, CRSP70, mediator of RNA polymerase II transcription subunit 26, ARC70, CRSP complex subunit 7, activator-recruited cofactor 70 kDa component, cofactor required for Sp1 transcriptional activation subunit 7, cofactor required for Sp1 transcriptional activation, subunit 7 (70kD), cofactor required for Sp1 transcriptional activation, subunit 7, 70kDa, transcriptional coactivator CRSP70
  • Host
    Rabbit
  • Protein IDO95402
  • Protein Name
    Mediator of RNA polymerase II transcription subunit 26
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of MED26 using anti-MED26 antibody (A09340-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MED26 antigen affinity purified polyclonal antibody (Catalog # A09340-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MED26 at approximately 65 kDa. The expected band size for MED26 is at 65 kDa.
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