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Western Blot analysis of JK cells using Med8 Polyclonal Antibody
Western Blot analysis of JK cells using Med8 Polyclonal Antibody
Western Blot analysis of JK cells using Med8 Polyclonal Antibody

MED8 Antibody

CSB-PA006810
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMED8
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Overview

  • Supplier
    Cusabio
  • Product Name
    MED8 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID112950
  • Target name
    MED8
  • Target description
    mediator complex subunit 8
  • Target synonyms
    ARC32, mediator of RNA polymerase II transcription subunit 8, activator-recruited cofactor 32 kDa component, mediator of RNA polymerase II transcription subunit MED8
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96G25
  • Protein Name
    Mediator of RNA polymerase II transcription subunit 8
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MED8 using anti-MED8 antibody (A08094-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MED8 antigen affinity purified polyclonal antibody (Catalog # A08094-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MED8 at approximately 29 kDa. The expected band size for MED8 is at 29 kDa.
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