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Western Blot analysis of HeLa cells using MEF-2 Polyclonal Antibody
Western Blot analysis of HeLa cells using MEF-2 Polyclonal Antibody
Western Blot analysis of HeLa cells using MEF-2 Polyclonal Antibody

MEF2A Antibody

CSB-PA003214
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMEF2A
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Overview

  • Supplier
    Cusabio
  • Product Name
    MEF2A Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4205
  • Target name
    MEF2A
  • Target description
    myocyte enhancer factor 2A
  • Target synonyms
    ADCAD1, RSRFC4, RSRFC9, mef2, myocyte-specific enhancer factor 2A, MADS box transcription enhancer factor 2, polypeptide A (myocyte enhancer factor 2A), serum response factor-like protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ02078
  • Protein Name
    Myocyte-specific enhancer factor 2A
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MEF2A using anti-MEF2A antibody (PB9953). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: HELA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEF2A antigen affinity purified polyclonal antibody (Catalog # PB9953) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEF2A at approximately 56 kDa. The expected band size for MEF2A is at 56 kDa.
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