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Western Blot analysis of COLO205 cells using EGFL5 Polyclonal Antibody
Western Blot analysis of COLO205 cells using EGFL5 Polyclonal Antibody
Western Blot analysis of COLO205 cells using EGFL5 Polyclonal Antibody

MEGF9 Antibody

CSB-PA002263
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMEGF9
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Overview

  • Supplier
    Cusabio
  • Product Name
    MEGF9 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1955
  • Target name
    MEGF9
  • Target description
    multiple EGF like domains 9
  • Target synonyms
    EGFL5, multiple epidermal growth factor-like domains protein 9, EGF-like-domain, multiple 5, epidermal growth factor-like protein 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H1U4
  • Protein Name
    Multiple epidermal growth factor-like domains protein 9
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MEGF9 using anti-MEGF9 antibody (A15544-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEGF9 antigen affinity purified polyclonal antibody (Catalog # A15544-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEGF9 at approximately 63 kDa. The expected band size for MEGF9 is at 63 kDa.
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