More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/MEK1 antibody [1F5]
WB analysis of wild type and MEK1 knock out HeLa cell lysate(10microg per lane) using GTX84167 MEK1 antibody [1F5]. Dilution : 1:500
WB analysis of wild type and MEK1 knock out HeLa cell lysate(10microg per lane) using GTX84167 MEK1 antibody [1F5]. Dilution : 1:500
WB analysis of wild type and MEK1 knock out HeLa cell lysate(10microg per lane) using GTX84167 MEK1 antibody [1F5]. Dilution : 1:500

MEK1 antibody [1F5]

GTX84167
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetMAP2K1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    MEK1 antibody [1F5] - KO/KD, Overexpression Validated
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:200-1:1000. ICC/IF: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1F5
  • Concentration
    0.99 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5604
  • Target name
    MAP2K1
  • Target description
    mitogen-activated protein kinase kinase 1
  • Target synonyms
    CFC3, MAPKK1, MEK1, MEL, MKK1, PRKMK1, dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAPK/ERK kinase 1, MAPKK 1, MEK 1, protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ02750
  • Protein Name
    Dual specificity mitogen-activated protein kinase kinase 1
  • Scientific Description
    Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Western Blot analysis of HuvEc cells using MEK-1 Polyclonal Antibody
Product group Antibodies
Cusabio
MAP2K1 AntibodyCSB-PA003217
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
IHC-P analysis of human kidney tissue using GTX25612 MEK1 (phospho Thr292) antibody. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:100
Product group Antibodies
GeneTex
MEK1 (phospho Thr292) antibodyGTX25612
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetMAP2K1
  • SizePrice
IHC-P analysis of human colon carcinoma tissue using GTX25613 MEK1 (phospho Ser298) antibody. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:100
Product group Antibodies
References
GeneTex
MEK1 (phospho Ser298) antibodyGTX25613
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetMAP2K1
  • SizePrice
WB (peptide competition) analysis of NIH3T3 cells treated with 50ng/mL PDGF for 15 minutes (Lane 2-5) using GTX25615 MEK1 (phospho Thr386) antibody with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphothreonine-containing peptide (Lane 4), or the phosphopeptide immunogen (Lane 5) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
Product group Antibodies
GeneTex
MEK1 (phospho Thr386) antibodyGTX25615
ApplicationsWestern Blot
TargetMAP2K1
  • SizePrice
WB analysis of PMA-treated HEK293T (A), UV-treated NIH3T3 (B), C2C12 (C), PMA-treated PC12 (D), C6 (E) whole cell lysates using GTX32248 MEK1 (phospho Thr286) antibody.
Product group Antibodies
GeneTex
MEK1 (phospho Thr286) antibodyGTX32248
ApplicationsWestern Blot
TargetMAP2K1
  • SizePrice
ICC/IF analysis of formalin-fixed HeLa cells using GTX32249 MEK1 (phospho Thr292) antibody. Red : Primary antibody Blue : DAPI Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
Product group Antibodies
GeneTex
MEK1 (phospho Thr292) antibodyGTX32249
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetMAP2K1
  • SizePrice
Product group Antibodies
GeneTex
MEK1 + MEK2 antibodyGTX111338
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetMAP2K1
  • SizePrice
IHC-P analysis of formalin fixed human colon cancer tissue section using GTX54968 MEK1/2 (phospho Ser218/Ser222) antibody. Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)
Product group Antibodies
GeneTex
MEK1/2 (phospho Ser218/Ser222) antibodyGTX54968
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetMAP2K1
  • SizePrice
ICC/IF analysis of formalin-fixed K562 cells using GTX54969 MEK1/2 (phospho Ser222/Ser226) antibody. Red : Primary antibody Blue : DAPI Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
Product group Antibodies
GeneTex
MEK1/2 (phospho Ser222/Ser226) antibodyGTX54969
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetMAP2K1
  • SizePrice
ICC/IF analysis of C6 cells using GTX01293 MEK1 antibody [GT1158]. Orange: Primary antibody Blue: DAPI
Product group Antibodies
GeneTex
MEK1 antibody [GT1158]GTX01293
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetMAP2K1
  • SizePrice