WB (peptide competition) analysis of NIH3T3 cells treated with 50 ng/mL PDGF for 15 minutes (Lane 2-5) using GTX24750 MEK1 + MEK2 (phospho Ser222) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphoserine-containing peptide (Lane 4), or the phosphopeptide immunogen (Lane 5) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
WB (peptide competition) analysis of NIH3T3 cells treated with 50 ng/mL PDGF for 15 minutes (Lane 2-5) using GTX24750 MEK1 + MEK2 (phospho Ser222) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphoserine-containing peptide (Lane 4), or the phosphopeptide immunogen (Lane 5) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
MEK1 + MEK2 (phospho Ser222) antibody
GTX24750
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
Overview
- SupplierGeneTex
- Product NameMEK1 + MEK2 (phospho Ser222) antibody - Orthogonal Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1,000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- FormulationLiquid
- HostRabbit
- IsotypeIgG
- Modification TypePhosphorylated
- Scientific DescriptionMicrotubule-associated protein kinase kinase 1 + 2 (MEK 1 + 2 or MAPKK 1+ 2) are members of a tyrosine/threonine protein kinase family that activate the ERK 1 + 2/MAPK enzymes by phosphorylating both residues within the threonine - glutamic acid - tyrosine (TEY) motif in the activation loop. MEK 1 + 2 are also activated by dual-phosphorylation, which occurs on serines 218 and 222, in the activation loop of the MEKs.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203