![Untreated (-) and treated (+) HeLa whole cell extracts (30 microg) were separated by 10% SDS-PAGE, and the membrane was blotted with MEK1 (phospho Ser298) antibody [GT1305] (GTX03217) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Untreated (-) and treated (+) HeLa whole cell extracts (30 microg) were separated by 10% SDS-PAGE, and the membrane was blotted with MEK1 (phospho Ser298) antibody [GT1305] (GTX03217) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX03217/GTX03217_4000001565_20210716_WB_CIP_w_23053123_880.webp)
Untreated (-) and treated (+) HeLa whole cell extracts (30 microg) were separated by 10% SDS-PAGE, and the membrane was blotted with MEK1 (phospho Ser298) antibody [GT1305] (GTX03217) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
MEK1 (phospho Ser298) antibody [GT1305]
GTX03217
ApplicationsWestern Blot
Product group Antibodies
TargetMAP2K1
Overview
- SupplierGeneTex
- Product NameMEK1 (phospho Ser298) antibody [GT1305] - Orthogonal Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT1305
- Concentration1.08 mg/ml
- ConjugateUnconjugated
- Gene ID5604
- Target nameMAP2K1
- Target descriptionmitogen-activated protein kinase kinase 1
- Target synonymsCFC3; dual specificity mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAPK/ERK kinase 1; MAPKK 1; MAPKK1; MEK 1; MEK1; MEL; MKK1; PRKMK1; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
- HostRabbit
- IsotypeIgG
- Protein IDQ02750
- Protein NameDual specificity mitogen-activated protein kinase kinase 1
- Scientific DescriptionThe protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008]
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![WB analysis of HeLa cells were treated by CIP(20uL/400ul) at 37oC for 1 hour or treated by PMA (200 nM) at 37oC for 30 minutes after serum-starvation overnight using GTX03217 MEK1 (phospho Ser298) antibody [GT1305]. Dilution : 1:1000 Loading : 25microg per lane WB analysis of HeLa cells were treated by CIP(20uL/400ul) at 37oC for 1 hour or treated by PMA (200 nM) at 37oC for 30 minutes after serum-starvation overnight using GTX03217 MEK1 (phospho Ser298) antibody [GT1305]. Dilution : 1:1000 Loading : 25microg per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX03217/GTX03217_82_WB_w_23053123_425.webp)
![WB analysis of C6 cells were treated by CIP(20uL/400ul) at 37oC for 1 hour or treated by PMA (200 nM) at 37oC for 30 minutes after serum-starvation overnight using GTX03217 MEK1 (phospho Ser298) antibody [GT1305]. Dilution : 1:1000 Loading : 25microg per lane WB analysis of C6 cells were treated by CIP(20uL/400ul) at 37oC for 1 hour or treated by PMA (200 nM) at 37oC for 30 minutes after serum-starvation overnight using GTX03217 MEK1 (phospho Ser298) antibody [GT1305]. Dilution : 1:1000 Loading : 25microg per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX03217/GTX03217_83_WB_w_23053123_506.webp)



![WB analysis of wild type and MEK1 knock out HeLa cell lysate(10microg per lane) using GTX84167 MEK1 antibody [1F5]. Dilution : 1:500](https://www.genetex.com/upload/website/prouct_img/normal/GTX84167/GTX84167_4170_20181228_WB_w_23061420_686.webp)
![WB analysis of HEK293T cells transfected with MEK1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84168 MEK1 antibody [1C1]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84168/GTX84168_4172_WB_w_23061420_362.webp)
![ICC/IF analysis of C6 cells using GTX01293 MEK1 antibody [GT1158]. Orange: Primary antibody Blue: DAPI](https://www.genetex.com/upload/website/prouct_img/normal/GTX01293/GTX01293_20191101_AP_002_69_w_23053121_682.webp)