Bio-Connect

MEK1 (phospho Thr291) antibody

GTX79017
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetMAP2K1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    GeneTex
  • Product Name
    MEK1 (phospho Thr291) antibody - Orthogonal Validated
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5604
  • Target name
    MAP2K1
  • Target description
    mitogen-activated protein kinase kinase 1
  • Target synonyms
    CFC3; dual specificity mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAPK/ERK kinase 1; MAPKK 1; MAPKK1; MEK 1; MEK1; MEL; MKK1; PRKMK1; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ02750
  • Protein Name
    Dual specificity mitogen-activated protein kinase kinase 1
  • Scientific Description
    The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
Biomatik
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetMAP2K1
  • SizePrice
Paraformaldehyde-fixed, paraffin embedded human lung carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-MEK1/2 Polyclonal Antibody, Unconjugated (bs-1041R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Product group Antibodies
References
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetMAP2K1
  • SizePrice
Product group Antibodies
Assay Genie
TargetMAP2K1
  • SizePrice
Product group Antibodies
GeneTex
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetMAP2K1
  • SizePrice
Product group Antibodies
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetMAP2K1
  • SizePrice
Western blot of human T47D cells showing phospho-specific immunolabeling of the ~45k MEK1 protein phosphorylated at Thr 386 using MEK1 (phospho Thr386) antibody (GTX82656).
Product group Antibodies
GeneTex
ApplicationsWestern Blot
TargetMAP2K1
  • SizePrice
Western blots of recombinant wild type and mutant MEK 1 showing phospho-specific immunolabeling. Lanes 1 and 2 are wild type MEK 1; lanes 3 and 4 are mutant MEK 1 (Thr 200 Arg). MAP kinase was co-expressed in the samples run in lanes 2 and 4. MEK1 (phospho Thr292) antibody was used (GTX82657).
Product group Antibodies
GeneTex
ApplicationsWestern Blot
TargetMAP2K1
  • SizePrice
WB analysis of wild type and MEK1 knock out HeLa cell lysate(10microg per lane) using GTX84167 MEK1 antibody [1F5]. Dilution : 1:500
Product group Antibodies
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetMAP2K1
  • SizePrice
WB analysis of HEK293T cells transfected with MEK1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84168 MEK1 antibody [1C1]. Loading : 5 ug per lane
Product group Antibodies
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetMAP2K1
  • SizePrice
IHC-P analysis of human kidney tissue using GTX25612 MEK1 (phospho Thr292) antibody. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:100
Product group Antibodies
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetMAP2K1
  • SizePrice