Untreated (-) and treated (+) HeLa whole cell extracts (30 microg) were separated by 10% SDS-PAGE, and the membrane was blotted with MEK1 (phospho Thr292) antibody [GT1306] (GTX03218) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![WB analysis of C6 cells were treated by Nocodazole (50 ng/mL) at 37oC for 20 hours using GTX03218 MEK1 (phospho Thr292) antibody [GT1306]. Dilution : 1:1000 Loading : 25microg per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX03218/GTX03218_84_WB_w_23053123_758.webp "WB analysis of C6 cells were treated by Nocodazole (50 ng/mL) at 37oC for 20 hours using GTX03218 MEK1 (phospho Thr292) antibody [GT1306]. Dilution : 1:1000 Loading : 25microg per lane")
![WB analysis of HeLa cells were treated by nocodazole (50 ng/mL) at 37oC for 20 hours using GTX03218 MEK1 (phospho Thr292) antibody [GT1306]. Dilution : 1:1000 Loading : 25microg per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX03218/GTX03218_85_WB_w_23053123_391.webp "WB analysis of HeLa cells were treated by nocodazole (50 ng/mL) at 37oC for 20 hours using GTX03218 MEK1 (phospho Thr292) antibody [GT1306]. Dilution : 1:1000 Loading : 25microg per lane")
Untreated (-) and treated (+) HeLa whole cell extracts (30 microg) were separated by 10% SDS-PAGE, and the membrane was blotted with MEK1 (phospho Thr292) antibody [GT1306] (GTX03218) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
MEK1 (phospho Thr292) antibody [GT1306] - Orthogonal Validated
GTX03218
Overview
- SupplierGeneTex
- Product NameMEK1 (phospho Thr292) antibody [GT1306] - Orthogonal Validated
- Delivery Days Customer9
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT1306
- Concentration1.14 mg/ml
- ConjugateUnconjugated
- Gene ID5604
- Target nameMAP2K1
- Target descriptionmitogen-activated protein kinase kinase 1
- Target synonymsCFC3; dual specificity mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAPK/ERK kinase 1; MAPKK 1; MAPKK1; MEK 1; MEK1; MEL; MKK1; PRKMK1; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
- HostRabbit
- IsotypeIgG
- Modification TypePhosphorylated
- Scientific DescriptionThe protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008]
- ReactivityHuman, Rat
- Storage Instruction2°C to 8°C,-20°C or -80°C
- UNSPSC12352203