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Phospho-MEK1/2 (Ser218 + Ser222) Rabbit pAb

ORB14908
Biorbyt
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetMAP2K2
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Overview

  • Supplier
    Biorbyt
  • Product Name
    MEK1/2 (phospho-Ser218/222) antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB=1:1000-2000, ELISA=1:5000-10000 ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5605
  • Target name
    MAP2K2
  • Target description
    mitogen-activated protein kinase kinase 2
  • Target synonyms
    CFC4; dual specificity mitogen-activated protein kinase kinase 2; ERK activator kinase 2; MAP kinase kinase 2; MAPK/ERK kinase 2; MAPKK2; MEK2; mitogen-activated protein kinase kinase 2, p45; MKK2; PRKMK2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP36507
  • Protein Name
    Dual specificity mitogen-activated protein kinase kinase 2
  • Scientific Description
    Phospho-MEK1/2 (Ser218 + Ser222) Rabbit pAb
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MEK2/MAP2K2 using anti-MEK2/MAP2K2 antibody (A00996-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hek293 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat lung tissue lysates, Lane 6: mouse ANA-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEK2/MAP2K2 antigen affinity purified polyclonal antibody (Catalog # A00996-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEK2/MAP2K2 at approximately 43KD. The expected band size for MEK2/MAP2K2 is at 43KD.
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