More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/MEK2 (MAP2K2) Mouse Monoclonal Antibody [Clone ID: OTI8G6]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MAP2K2 (Cat# RC201827, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K2(Cat# TA500471). Positive lysates [LY403069] (100ug) and [LC403069] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MAP2K2 (Cat# RC201827, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K2(Cat# TA500471). Positive lysates [LY403069] (100ug) and [LC403069] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MAP2K2 (Cat# RC201827, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K2(Cat# TA500471). Positive lysates [LY403069] (100ug) and [LC403069] (20ug) can be purchased separately from OriGene.

MEK2 (MAP2K2) Mouse Monoclonal Antibody [Clone ID: OTI8G6]

CF500471
OriGene
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMAP2K2
Price on request
100 ug
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    OriGene
  • Product Name
    MEK2 (MAP2K2) Mouse Monoclonal Antibody [Clone ID: OTI8G6]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI8G6
  • Gene ID5605
  • Target name
    MAP2K2
  • Target description
    mitogen-activated protein kinase kinase 2
  • Target synonyms
    CFC4, MAPKK2, MEK2, MKK2, PRKMK2, dual specificity mitogen-activated protein kinase kinase 2, ERK activator kinase 2, MAP kinase kinase 2, MAPK/ERK kinase 2, mitogen-activated protein kinase kinase 2, p45
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP36507
  • Protein Name
    Dual specificity mitogen-activated protein kinase kinase 2
  • Scientific Description
    Carrier-free (BSA/glycerol-free) MAP2K2 mouse monoclonal antibody, clone OTI8G6 (formerly 8G6)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

Download MSDS

FAQ

Go to our frequently asked questions

Related products

Anti-MEK2 Antibody
Product group Antibodies
Antibodies.com
Anti-MEK2 AntibodyA95356
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
Figure 1. Western blot analysis of MEK2/MAP2K2 using anti-MEK2/MAP2K2 antibody (A00996-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hek293 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat lung tissue lysates, Lane 6: mouse ANA-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEK2/MAP2K2 antigen affinity purified polyclonal antibody (Catalog # A00996-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEK2/MAP2K2 at approximately 43KD. The expected band size for MEK2/MAP2K2 is at 43KD.
Product group Antibodies
Boster Bio
Anti-MEK2/MAP2K2 Antibody Picoband(r)A00996-2-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP2K2
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
MAP2K2 / MKK2 / MEK2 Antibody (clone BNHE)LS-C768024
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAP2K2
  • SizePrice
Product group Antibodies
MedChem Express
MEK1/2 Antibody (YA299, Rabbit)HY-P80218
TargetMAP2K1
  • SizePrice
Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
Product group Antibodies
Atlas Antibodies
Anti-MAP2K2 AntibodyHPA051993
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetMAP2K2
  • SizePrice
Product group Antibodies
Everest Biotech
Goat anti-MAP2K2 / MEK2EB12289
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetMAP2K2
  • SizePrice
Western blot analysis of 1) Hela, 2)3T3, 3) Rat Brain Tissue with MEK2 Mouse mAb diluted at 1:2,000.
Product group Antibodies
Cusabio
MAP2K2 Monoclonal AntibodyCSB-MA671424
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP2K2
  • SizePrice
Product group Antibodies
Biomatik
MAP2K2 Polyclonal AntibodyCAC14653
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetMAP2K2
  • SizePrice
Product group Antibodies
Bioss
MEK1 Recombinant AntibodyBSM-60744R
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse
TargetMAP2K2
  • SizePrice
Wild-type (WT) and MEK2 knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with MEK2 antibody (GTX102186) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
GeneTex
MEK2 antibodyGTX102186
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetMAP2K2
  • SizePrice