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ProductsBack/MEK6 (MAP2K6) Rabbit Polyclonal Antibody
Western Blot analysis of extracts from MDA, 293 and HeLa cells using MKK6 antibody
Western Blot analysis of extracts from MDA, 293 and HeLa cells using MKK6 antibody
Western Blot analysis of extracts from MDA, 293 and HeLa cells using MKK6 antibody

MEK6 (MAP2K6) Rabbit Polyclonal Antibody

AP02676PU-N
OriGene
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetMAP2K6
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    MEK6 (MAP2K6) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID5608
  • Target name
    MAP2K6
  • Target description
    mitogen-activated protein kinase kinase 6
  • Target synonyms
    CRCMSL, MAPKK6, MEK6, MKK6, PRKMK6, SAPKK-3, SAPKK3, dual specificity mitogen-activated protein kinase kinase 6, Colorectal Cancer Metastasis-Suppressed LncRNA, MAPK/ERK kinase 6, MAPKK 6, MEK 6, SAPK kinase 3, protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6), stress-activated protein kinase kinase 3
  • Host
    Rabbit
  • Protein IDP52564
  • Protein Name
    Dual specificity mitogen-activated protein kinase kinase 6
  • Scientific Description
    MEK6 (MAP2K6) rabbit polyclonal antibody, Aff - Purified
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of MKK6/MAP2K6 using anti-MKK6/MAP2K6 antibody (A02011-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: rat skeletal muscle tissue lysates, Lane 3: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MKK6/MAP2K6 antigen affinity purified polyclonal antibody (Catalog # A02011-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MKK6/MAP2K6 at approximately 38 kDa. The expected band size for MKK6/MAP2K6 is at 38 kDa.
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