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WB analysis of extracts from rat brain tissue using GTX50801 MELK antibody.
WB analysis of extracts from rat brain tissue using GTX50801 MELK antibody.
WB analysis of extracts from rat brain tissue using GTX50801 MELK antibody.

MELK antibody

GTX50801
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetMELK
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Overview

  • Supplier
    GeneTex
  • Product Name
    MELK antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID9833
  • Target name
    MELK
  • Target description
    maternal embryonic leucine zipper kinase
  • Target synonyms
    HPK38; maternal embryonic leucine zipper kinase; pEg3 kinase; protein kinase Eg3; protein kinase PK38; tyrosine-protein kinase MELK
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14680
  • Protein Name
    Maternal embryonic leucine zipper kinase
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MELK using anti-MELK antibody (A04038-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human U87 whole cell lysates, Lane 5: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MELK antigen affinity purified polyclonal antibody (Catalog # A04038-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MELK at approximately 66 kDa. The expected band size for MELK is at 75 kDa.
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