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Western Blot Positive WB detected in: HEK293 whole cell lysate, Hela whole cell lysate All lanes: MELTF antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 81, 33 kDa Observed band size: 81 kDa
Western Blot Positive WB detected in: HEK293 whole cell lysate, Hela whole cell lysate All lanes: MELTF antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 81, 33 kDa Observed band size: 81 kDa
Western Blot Positive WB detected in: HEK293 whole cell lysate, Hela whole cell lysate All lanes: MELTF antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 81, 33 kDa Observed band size: 81 kDa

MELTF Antibody

CSB-PA013754LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMELTF
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Overview

  • Supplier
    Cusabio
  • Product Name
    MELTF Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4241
  • Target name
    MELTF
  • Target description
    melanotransferrin
  • Target synonyms
    CD228, MAP97, MFI2, MTF1, MTf, melanotransferrin, antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5, membrane-bound transferrin-like protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08582
  • Protein Name
    Melanotransferrin
  • Scientific Description
    Involved in iron cellular uptake. Seems to be internalized and then recycled back to the cell membrane. Binds a single atom of iron per subunit. Could also bind zinc.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MELTF using anti-MELTF antibody (A12988-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A375 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MELTF antigen affinity purified polyclonal antibody (Catalog # A12988-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MELTF at approximately 90 kDa. The expected band size for MELTF is at 80 kDa.
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