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Western Blot analysis of HuvEc cells using Menin Polyclonal Antibody
Western Blot analysis of HuvEc cells using Menin Polyclonal Antibody
Western Blot analysis of HuvEc cells using Menin Polyclonal Antibody

MEN1 Antibody

CSB-PA003227
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMEN1
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Overview

  • Supplier
    Cusabio
  • Product Name
    MEN1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4221
  • Target name
    MEN1
  • Target description
    menin 1
  • Target synonyms
    MEAI; menin; SCG2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00255
  • Protein Name
    Menin
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MEN1 using anti-MEN1 antibody (A00331-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEN1 antigen affinity purified polyclonal antibody (Catalog # A00331-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEN1 at approximately 70 kDa. The expected band size for MEN1 is at 63-70,75 kDa.
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