Affinity purified phospho-specific antibody to NF2 (Merlin) at pS518 (GTX22478) was used at a 1:1000 dilution to detect NF2 by Western blot. Approximately 12 ul of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock treated (lane 1) or CLA treated at 4nM, 20 nM or 100 nM (lanes 2, 3 and 4 respectively) for 45'. After washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG (GTX27090) preceded color development using Amersham's substrate system. Other detection methods will yield similar results.
Affinity purified phospho-specific antibody to NF2 (Merlin) at pS518 (GTX22478) was used at a 1:1000 dilution to detect NF2 by Western blot. Approximately 12 ul of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock treated (lane 1) or CLA treated at 4nM, 20 nM or 100 nM (lanes 2, 3 and 4 respectively) for 45'. After washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG (GTX27090) preceded color development using Amersham's substrate system. Other detection methods will yield similar results.
Merlin (phospho Ser518) antibody
GTX22478
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
Product group Antibodies
ReactivityHuman, Mouse
TargetNF2
Overview
- SupplierGeneTex
- Product NameMerlin (phospho Ser518) antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:2000. ELISA: 1:200000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID4771
- Target nameNF2
- Target descriptionNF2, moesin-ezrin-radixin like (MERLIN) tumor suppressor
- Target synonymsACN, BANF, SCH, SWNV, merlin-1, merlin, bilateral acoustic neurofibromatosis, moesin-ezrin-radixin like, moesin-ezrin-radixin-like protein, moesin-ezrin-radizin-like protein, neurofibromin 2 (bilateral acoustic neuroma), neurofibromin-2, schwannomerlin, schwannomin
- HostRabbit
- IsotypeIgG
- Protein IDP35240
- Protein NameMerlin
- Scientific DescriptionThe recently isolated neurofibromatosis 2 tumor suppressor gene encodes a 595 amino acid protein (Merlin). The protein product Merlin, named for its relatedness to the ezrin, radixin and moesin (ERM) family of proteins, is a tumor suppressor whose absence results in the occurrence of multiple tumors of the nervous system, particularly schwannomas and meningiomas. Merlins similarity to ERMs suggests that it might share functions, acting as a link between cytoskeletal components and the cell membrane. The NF2 protein is highly expressed in human fibroblasts and detected as a single band of about 75kD. This antibody is specific for the phosphorylated form of human NF2 (Merlin).
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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