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ProductsBack/MESP1 Antibody - N-terminal region (OAAB09387)

MESP1 Antibody - N-terminal region (OAAB09387)

OAAB09387
Aviva Systems Biology
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetMESP1
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    MESP1 Antibody - N-terminal region (OAAB09387)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Format
    Liquid. PBS with 0.09% (W/V) sodium azide.
  • Gene ID55897
  • Target name
    MESP1
  • Target description
    mesoderm posterior bHLH transcription factor 1
  • Target synonyms
    bHLHc5, mesoderm posterior protein 1, class C basic helix-loop-helix protein 5, mesoderm posterior 1 homolog, mesoderm posterior basic helix-loop-helix transcription factor 1
  • Host
    Rabbit
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C,-20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MESP1 using anti-MESP1 antibody (A07301-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human Raji whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MESP1 antigen affinity purified polyclonal antibody (Catalog # A07301-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MESP1 at approximately 29KD. The expected band size for MESP1 is at 29KD.
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