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Western Blot Positive WB detected in: Hela whole cell lysate, U87 whole cell lysate, K562 whole cell lysate, PC3 whole cell lysate, Mouse brain tissue, Rat kidney tissue, Rat brain tissue All lanes: METRNL antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 35, 26 KDa Observed band size: 35 KDa Exposure time : 1min
Western Blot Positive WB detected in: Hela whole cell lysate, U87 whole cell lysate, K562 whole cell lysate, PC3 whole cell lysate, Mouse brain tissue, Rat kidney tissue, Rat brain tissue All lanes: METRNL antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 35, 26 KDa Observed band size: 35 KDa Exposure time : 1min
Western Blot Positive WB detected in: Hela whole cell lysate, U87 whole cell lysate, K562 whole cell lysate, PC3 whole cell lysate, Mouse brain tissue, Rat kidney tissue, Rat brain tissue All lanes: METRNL antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 35, 26 KDa Observed band size: 35 KDa Exposure time : 1min

METRNL Antibody

CSB-PA731035LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMETRNL
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Overview

  • Supplier
    Cusabio
  • Product Name
    METRNL Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID284207
  • Target name
    METRNL
  • Target description
    meteorin like, glial cell differentiation regulator
  • Target synonyms
    meteorin-like protein, cometin, meteorin, glial cell differentiation regulator-like, subfatin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ641Q3
  • Protein Name
    Meteorin-like protein
  • Scientific Description
    Hormone induced following exercise or cold exposure that promotes energy expenditure. Induced either in the skeletal muscle after exercise or in adipose tissue following cold exposure and is present in the circulation. Able to stimulate energy expenditure associated with the browning of the white fat depots and improves glucose tolerance. Does not promote an increase in a thermogenic gene program via direct action on adipocytes, but acts by stimulating several immune cell subtypes to enter the adipose tissue and activate their prothermogenic actions. Stimulates an eosinophil-dependent increase in IL4 expression and promotes alternative activation of adipose tissue macrophages, which are required for the increased expression of the thermogenic and anti-inflammatory gene programs in fat. Required for some cold-induced thermogenic responses, suggesting a role in metabolic adaptations to cold temperatures.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Meteorin-like/METRNL using anti-Meteorin-like/METRNL antibody (A11383-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Meteorin-like/METRNL antigen affinity purified polyclonal antibody (Catalog # A11383-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Meteorin-like/METRNL at approximately 34 kDa. The expected band size for Meteorin-like/METRNL is at 34 kDa.
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