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ProductsBack/METTL1 antibody, Internal
WB analysis of HepG2 cells using GTX46207 METTL1 antibody at 2.5μg/ml. Lane A: marker Lane B: HepG2 cells
WB analysis of HepG2 cells using GTX46207 METTL1 antibody at 2.5μg/ml. Lane A: marker Lane B: HepG2 cells
WB analysis of HepG2 cells using GTX46207 METTL1 antibody at 2.5μg/ml. Lane A: marker Lane B: HepG2 cells

METTL1 antibody, Internal

GTX46207
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetMETTL1
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Overview

  • Supplier
    GeneTex
  • Product Name
    METTL1 antibody, Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.2-2.5 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4234
  • Target name
    METTL1
  • Target description
    methyltransferase 1, tRNA methylguanosine
  • Target synonyms
    C12orf1, TRM8, TRMT8, YDL201w, tRNA (guanine-N(7)-)-methyltransferase, D1075-like gene product, mRNA (guanine-N(7)-)-methyltransferase, methyltransferase like 1, methyltransferase-like protein 1, miRNA (guanine-N(7)-)-methyltransferase, tRNA (guanine(46)-N(7))-methyltransferase, tRNA(m7G46)-methyltransferase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UBP6
  • Protein Name
    tRNA (guanine-N(7)-)-methyltransferase
  • Scientific Description
    This gene is similar in sequence to the S. cerevisiae YDL201w gene. The gene product contains a conserved S-adenosylmethionine-binding motif and is inactivated by phosphorylation. Alternative splice variants encoding different protein isoforms have been described for this gene. A pseudogene has been identified on chromosome X. [provided by RefSeq, Jul 2008]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of METTL1 using anti-METTL1 antibody (A10859-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: human PC-3 whole cell lysates, Lane 7: human T-47D whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-METTL1 antigen affinity purified polyclonal antibody (Catalog # A10859-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for METTL1 at approximately 34KD. The expected band size for METTL1 is at 34KD.
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