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Paraformaldehyde-fixed, paraffin embedded mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with METTL11A Polyclonal Antibody (bs-6057R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with METTL11A Polyclonal Antibody (bs-6057R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with METTL11A Polyclonal Antibody (bs-6057R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.

METTL11A Polyclonal Antibody

BS-6057R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetNTMT1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    METTL11A Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID28989
  • Target name
    NTMT1
  • Target description
    N-terminal Xaa-Pro-Lys N-methyltransferase 1
  • Target synonyms
    AD-003; alpha N-terminal protein methyltransferase 1A; C9orf32; HOMT1A; methyltransferase-like protein 11A; METTL11A; NRMT; NRMT1; N-terminal RCC1 methyltransferase; N-terminal Xaa-Pro-Lys N-methyltransferase 1; NTM1A; X-Pro-Lys N-terminal protein methyltransferase 1A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BV86
  • Protein Name
    N-terminal Xaa-Pro-Lys N-methyltransferase 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of METTL11A/NTMT1 using anti-METTL11A/NTMT1 antibody (A10023-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-METTL11A/NTMT1 antigen affinity purified polyclonal antibody (Catalog # A10023-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for METTL11A/NTMT1 at approximately 25 kDa. The expected band size for METTL11A/NTMT1 is at 25 kDa.
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