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ProductsBack/MGAM / Maltase-Glucoamylase Antibody (aa213-392)

MGAM / Maltase-Glucoamylase Antibody (aa213-392)

LS-C295281
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetMGAM
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    MGAM / Maltase-Glucoamylase Antibody (aa213-392)
  • Delivery Days Customer
    23
  • Application Supplier Note
    ICC 5-20ug/mL; WB 0.5-5ug/mL;
  • Applications
    Western Blot
  • Applications Supplier
    WB (0.5 - 5 µg/ml) ICC 5-20ug/mL; WB 0.5-5ug/mL;
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8972
  • Target name
    MGAM
  • Target description
    maltase-glucoamylase
  • Target synonyms
    brush border hydrolase; maltase-glucoamylase (alpha-glucosidase); maltase-glucoamylase, intestinal; MG; MGA
  • Host
    Rabbit
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MGAM using anti-MGAM antibody (A07347). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat small intestine lysates, Lane 2: mouse small intestine lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MGAM antigen affinity purified polyclonal antibody (Catalog # A07347) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MGAM at approximately 240 kDa. The expected band size for MGAM is at 210 kDa.
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