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Western Blot analysis of Jurkat cells using MGMT Polyclonal Antibody
Western Blot analysis of Jurkat cells using MGMT Polyclonal Antibody
Western Blot analysis of Jurkat cells using MGMT Polyclonal Antibody

MGMT Antibody

CSB-PA003238
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetMGMT
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Overview

  • Supplier
    Cusabio
  • Product Name
    MGMT Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4255
  • Target name
    MGMT
  • Target description
    O-6-methylguanine-DNA methyltransferase
  • Target synonyms
    methylated-DNA--protein-cysteine methyltransferase, 6-O-methylguanine-DNA methyltransferase, O-6-methylguanine-DNA-alkyltransferase, O6-methylguanine-DNA methyltransferase, methylguanine-DNA methyltransferase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP16455
  • Protein Name
    Methylated-DNA--protein-cysteine methyltransferase
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MGMT using anti-MGMT antibody (PB9266). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MGMT antigen affinity purified polyclonal antibody (Catalog # PB9266) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MGMT at approximately 22 kDa. The expected band size for MGMT is at 22 kDa.
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