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Western blot All lanes: MICA antibody at 8ug/ml Lane 1: MCF-7 whole cell lysate Lane 2: A431 whole cell lysate Lane 3: Mouse Stomach tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 43, 32 kDa Observed band size: 43 kDa
Western blot All lanes: MICA antibody at 8ug/ml Lane 1: MCF-7 whole cell lysate Lane 2: A431 whole cell lysate Lane 3: Mouse Stomach tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 43, 32 kDa Observed band size: 43 kDa
Western blot All lanes: MICA antibody at 8ug/ml Lane 1: MCF-7 whole cell lysate Lane 2: A431 whole cell lysate Lane 3: Mouse Stomach tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 43, 32 kDa Observed band size: 43 kDa

MICA Antibody

CSB-PA013806ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetMICA
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Overview

  • Supplier
    Cusabio
  • Product Name
    MICA Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID100507436
  • Target name
    MICA
  • Target description
    MHC class I polypeptide-related sequence A
  • Target synonyms
    MIC-A, PERB11.1, MHC class I polypeptide-related sequence A, HLA class I antigen, MHC class I chain-related protein A, MHC class I related chain A, MHC class I related sequence A, major histocompatibility complex class I chain-related protein A, stress inducible class I homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ29983
  • Protein Name
    MHC class I polypeptide-related sequence A
  • Scientific Description
    Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T-cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MICA using anti-MICA antibody (PB9612). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human SGC-7901 whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: huamn Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MICA antigen affinity purified polyclonal antibody (Catalog # PB9612) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MICA at approximately 45 kDa. The expected band size for MICA is at 43 kDa.
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