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ProductsBack/MICB Polyclonal Antibody
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-MICB Polyclonal Antibody, Unconjugated (bs-6933R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-MICB Polyclonal Antibody, Unconjugated (bs-6933R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-MICB Polyclonal Antibody, Unconjugated (bs-6933R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

MICB Polyclonal Antibody

BS-6933R
Bioss
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetMICB
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Overview

  • Supplier
    Bioss
  • Product Name
    MICA+MICB Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID4277
  • Target name
    MICB
  • Target description
    MHC class I polypeptide-related sequence B
  • Target synonyms
    PERB11.2, MHC class I polypeptide-related sequence B, MHC class I antigen-related protein B, MHC class I chain-related protein B, MHC class I mic-B antigen, MHC class I-like molecule PERB11.2-IMX, stress inducible class I homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ29980
  • Protein Name
    MHC class I polypeptide-related sequence B
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • The High Expression of the microRNA 17-92 Cluster and its Paralogs, and the Downregulation of the Target Gene PTEN, Is Associated with Primary Cutaneous B-Cell Lymphoma Progression. Battistella M et al., 2015 Jun, J Invest Dermatol
    Read more

Datasheet

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Figure 1. Western blot analysis of MICB using anti-MICB antibody (PB9613). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: human 293T whole cell lysates, Lane 6: human SiHa whole cell lysates, Lane 7: human HEPA1/6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MICB antigen affinity purified polyclonal antibody (Catalog # PB9613) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MICB at approximately 60-65 kDa. The expected band size for MICB is at 43 kDa.
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Immunofluorescent staining of human cell line SiHa shows localization to the Golgi apparatus & vesicles.
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