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ProductsBack/Microsomal Glutathione S Transferase 1 (MGST1) Polyclonal Antibody

Microsomal Glutathione S Transferase 1 (MGST1) Polyclonal Antibody

CAU37067
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetMGST1
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Overview

  • Supplier
    Biomatik
  • Product Name
    Microsomal Glutathione S Transferase 1 (MGST1) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4257
  • Target name
    MGST1
  • Target description
    microsomal glutathione S-transferase 1
  • Target synonyms
    glutathione S-transferase 12; GST12; MGST; MGST-I; microsomal glutathione S-transferase 1; microsomal GST-1; microsomal GST-I
  • Host
    Rabbit
  • Protein IDP10620
  • Protein Name
    Microsomal glutathione S-transferase 1
  • Scientific Description
    The Microsomal Glutathione S Transferase 1 (MGST1) Polyclonal Antibody (Species: Human) has been validated for the following applications: WB, IHC, ICC, IP.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MGST1 using anti-MGST1 antibody (PB9723). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Liver Tissue Lysate at 50ug, Lane 2: Mouse Liver Tissue Lysate at 50ug, Lane 3: HEPG2 Whole Cell Lysate at 40ug. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MGST1 antigen affinity purified polyclonal antibody (Catalog # PB9723) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MGST1 at approximately 17KD. The expected band size for MGST1 is at 17KD.
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