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MIF antibody [2Ar3]

GTX14575
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetMIF
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Overview

  • Supplier
    GeneTex
  • Product Name
    MIF antibody [2Ar3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2Ar3
  • Conjugate
    Unconjugated
  • Gene ID4282
  • Target name
    MIF
  • Target description
    macrophage migration inhibitory factor
  • Target synonyms
    epididymis secretory sperm binding protein; GIF; GLIF; L-dopachrome isomerase; L-dopachrome tautomerase; macrophage migration inhibitory factor; macrophage migration inhibitory factor (glycosylation-inhibiting factor); MMIF; phenylpyruvate tautomerase
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP14174
  • Protein Name
    Macrophage migration inhibitory factor
  • Scientific Description
    This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Western blot analysis of GeneTexs IgY fraction of Chicken-anti-Human MIF polyclonal antibody shows the detection of 100 microg of recombinant MIF present in a lysate. Similar detection of MIF will occur when human serum is analyzed. In lane 1 no reaction is observed in the control whereas lane 2 shows a single band at 12.3 kDa. A 4-20% gradient gel was used to separate the proteins by SDS-PAGE. The protein was transferred to nitro-cellulose using standard methods. After blocking the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:5,000 dilution of IRDye800 conjugated Gt-a-Chicken Rabbit IgG [H&L] for 1 h at room temperature. LICORs OdysseyR Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
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