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Western blot All lanes: MIOX antibody at 6microg/ml + Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 34, 25 kDa Observed band size: 34 kDa
Western blot All lanes: MIOX antibody at 6microg/ml + Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 34, 25 kDa Observed band size: 34 kDa
Western blot All lanes: MIOX antibody at 6microg/ml + Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 34, 25 kDa Observed band size: 34 kDa

MIOX Antibody

CSB-PA866220LA01HU
Cusabio
ApplicationsImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetMIOX
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Overview

  • Supplier
    Cusabio
  • Product Name
    MIOX Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoPrecipitation, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55586
  • Target name
    MIOX
  • Target description
    myo-inositol oxygenase
  • Target synonyms
    ALDRL6, inositol oxygenase, MI oxygenase, aldehyde reductase (aldose reductase) like 6, aldehyde reductase-like 6, kidney-specific protein 32, renal-specific oxidoreductase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UGB7
  • Protein Name
    Inositol oxygenase
  • Scientific Description
    cytoplasm, extracellular exosome, inclusion body, aldo-keto reductase (NADP) activity, ferric iron binding, inositol oxygenase activity, oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, inositol catabolic process
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MIOX using anti-MIOX antibody (A06957). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MIOX antigen affinity purified polyclonal antibody (Catalog # A06957) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MIOX at approximately 36 kDa. The expected band size for MIOX is at 33 kDa.
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