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Rat MIP-1 alpha detected by Western blot in RAW264.7 cells treated with LPS (15 hr). 1. Lysate 2. Supernatant
Rat MIP-1 alpha detected by Western blot in RAW264.7 cells treated with LPS (15 hr). 1. Lysate 2. Supernatant
Rat MIP-1 alpha detected by Western blot in RAW264.7 cells treated with LPS (15 hr). 1. Lysate 2. Supernatant

MIP1 alpha antibody

GTX27738
GeneTex
ApplicationsImmunoPrecipitation, Western Blot
DownloadDatasheet
Product group Antibodies
TargetCcl3
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Overview

  • Supplier
    GeneTex
  • Product Name
    MIP1 alpha antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    For WB: Use at a dilution of 1:1,500 - 1:2,500. For IP: Use at a dilution of 1:400 - 1:600. Optimal dilutions/concentrations should be determined by the researcher.
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID25542
  • Target name
    Ccl3
  • Target description
    C-C motif chemokine ligand 3
  • Target synonyms
    C-C motif chemokine 3; chemokine (C-C motif) ligand 3; Macrophage inflammatory protein 1 alpha (Small inducible cytokine A3); macrophage inflammatory protein 1-alpha; MIP-1a; MIP-1-alpha; Scya3; small-inducible cytokine A3
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CCL3 using anti-CCL3 antibody (PB9022). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant Rat CCL3 Protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCL3 antigen affinity purified polyclonal antibody (Catalog # PB9022) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCL3 at approximately 33KD. The expected band size for CCL3 is at 33KD.
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