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MIS18A / B28 Antibody

LS-C754984
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetMIS18A
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    MIS18A / B28 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Applications Supplier
    ELISA, IF (1:20 - 1:200), WB Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID54069
  • Target name
    MIS18A
  • Target description
    MIS18 kinetochore protein A
  • Target synonyms
    B28, C21orf45, C21orf46, FASP1, MIS18alpha, hMis18alpha, protein Mis18-alpha, FAPP1-associated protein 1, MIS18 kinetochore protein homolog A
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of C21orf45/MIS18A using anti-C21orf45/MIS18A antibody (A10791-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C21orf45/MIS18A antigen affinity purified polyclonal antibody (Catalog # A10791-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C21orf45/MIS18A at approximately 26 kDa. The expected band size for C21orf45/MIS18A is at 26 kDa.
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