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Mitochondrial Uncoupling Protein 2 (UCP2) Polyclonal Antibody

CAU24089
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetUCP2
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Overview

  • Supplier
    Biomatik
  • Product Name
    Mitochondrial Uncoupling Protein 2 (UCP2) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7351
  • Target name
    UCP2
  • Target description
    uncoupling protein 2
  • Target synonyms
    BMIQ4, SLC25A8, UCPH, dicarboxylate carrier SLC25A8, mitochondrial uncoupling protein 2, solute carrier family 25 member 8, uncoupling protein 2 (mitochondrial, proton carrier)
  • Host
    Rabbit
  • Protein IDP55851
  • Protein Name
    Dicarboxylate carrier SLC25A8
  • Scientific Description
    The Mitochondrial Uncoupling Protein 2 (UCP2) Polyclonal Antibody (Species: Human) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of UCP2 using anti-UCP2 antibody (A02256-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysate,Lane 2: rat cardiac muscle tissue lysate,Lane 3: rat brain tissue lysate,Lane 4: mouse spleen tissue lysate,Lane 5: mouse cardiac muscle tissue lysate,Lane 6: mouse brain tissue lysate,Lane 7: human Hela whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UCP2 antigen affinity purified polyclonal antibody (Catalog # A02256-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UCP2 at approximately 33KD. The expected band size for UCP2 is at 33KD.
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