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WB analysis of various sample lysates using GTX33318 Mitofilin antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX33318 Mitofilin antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX33318 Mitofilin antibody. Dilution : 1:1000 Loading : 25microg per lane

Mitofilin antibody

GTX33318
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetIMMT
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Overview

  • Supplier
    GeneTex
  • Product Name
    Mitofilin antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. ICC/IF: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10989
  • Target name
    IMMT
  • Target description
    inner membrane mitochondrial protein
  • Target synonyms
    cell proliferation-inducing gene 4/52 protein; cell proliferation-inducing protein 52; heart muscle protein; HMP; Mic60; MICOS complex subunit MIC60; MICOS60; MINOS2; mitochondrial contact site and cristae organizing system subunit 60; mitochondrial inner membrane organizing system 2; mitochondrial inner membrane protein; mitofilin; motor protein; P87; P87/89; P89; PIG4; PIG52; proliferation-inducing gene 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16891
  • Protein Name
    MICOS complex subunit MIC60
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Mitofilin/IMMT using anti-Mitofilin/IMMT antibody (A04102-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Colo320 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat NRK whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse HEPA1/6 whole cell lysates, Lane 7: mouse MFC whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mitofilin/IMMT antigen affinity purified polyclonal antibody (Catalog # A04102-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mitofilin/IMMT at approximately 80-90 kDa. The expected band size for Mitofilin/IMMT is at 84 kDa.
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