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Mitofilin Polyclonal Antibody, Cy5.5 Conjugated

BS-1824R-CY5.5
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Human, Mouse, Rat
TargetIMMT
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Overview

  • Supplier
    Bioss
  • Product Name
    Mitofilin Polyclonal Antibody, Cy5.5 Conjugated
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Cy5.5
  • Gene ID10989
  • Target name
    IMMT
  • Target description
    inner membrane mitochondrial protein
  • Target synonyms
    HMP, MICOS60, MINOS2, Mic60, P87, P87/89, P89, PIG4, PIG52, MICOS complex subunit MIC60, cell proliferation-inducing gene 4/52 protein, cell proliferation-inducing protein 52, heart muscle protein, mitochondrial contact site and cristae organizing system subunit 60, mitochondrial inner membrane organizing system 2, mitochondrial inner membrane protein, mitofilin, motor protein, proliferation-inducing gene 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16891
  • Protein Name
    MICOS complex subunit MIC60
  • Reactivity
    Bovine, Canine, Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA598310(IMMT Antibody) at dilution 1/12, on the right is treated with fusion protein. (Original magnification: x200)
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Figure 1. Western blot analysis of Mitofilin/IMMT using anti-Mitofilin/IMMT antibody (A04102-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Colo320 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat NRK whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse HEPA1/6 whole cell lysates, Lane 7: mouse MFC whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mitofilin/IMMT antigen affinity purified polyclonal antibody (Catalog # A04102-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mitofilin/IMMT at approximately 80-90 kDa. The expected band size for Mitofilin/IMMT is at 84 kDa.
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Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Mitofilin/IMMT Polyclonal Antibody, Unconjugated (bs-1824R) at 1:400 overnight at 4°C, DAB staining.
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GeneTex
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