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Western Blot analysis of HeLa cells using MRTF-A Polyclonal Antibody
Western Blot analysis of HeLa cells using MRTF-A Polyclonal Antibody
Western Blot analysis of HeLa cells using MRTF-A Polyclonal Antibody

MKL1 Antibody

CSB-PA003319
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetMRTFA
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Overview

  • Supplier
    Cusabio
  • Product Name
    MKL1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57591
  • Target name
    MRTFA
  • Target description
    myocardin related transcription factor A
  • Target synonyms
    BSAC, MAL, MKL, MKL1, MRTF-A, myocardin-related transcription factor A, MKL/myocardin-like protein 1, alternative protein MKL1, basic, SAP and coiled-coil domain, megakaryoblastic leukemia (translocation) 1, megakaryoblastic leukemia 1 protein, megakaryocytic acute leukemia protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ969V6
  • Protein Name
    MKL/myocardin-like protein 1
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Mkl1/MRTFA using anti-Mkl1/MRTFA antibody (A32468-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mkl1/MRTFA antigen affinity purified polyclonal antibody (Catalog # A32468-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mkl1/MRTFA at approximately 145 kDa. The expected band size for Mkl1/MRTFA is at 145 kDa.
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