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Lane 1: A549 cell lysates; Lane 2: Hela cell lysates probed with _x000D_ MKLP1 (7C9) Monoclonal Antibody (bsm-52401R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37˚C.
Lane 1: A549 cell lysates; Lane 2: Hela cell lysates probed with _x000D_ MKLP1 (7C9) Monoclonal Antibody (bsm-52401R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37˚C.
Lane 1: A549 cell lysates; Lane 2: Hela cell lysates probed with _x000D_ MKLP1 (7C9) Monoclonal Antibody (bsm-52401R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37˚C.

MKLP1 Recombinant Antibody

BSM-52401R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetKIF23
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Overview

  • Supplier
    Bioss
  • Product Name
    MKLP1 Recombinant Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Applications Supplier
    WB(WB=1:500-1000), Flow-Cyt(Flow-Cyt=2ug/Test), ICC/IF(ICC/IF=1:50)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID9493
  • Target name
    KIF23
  • Target description
    kinesin family member 23
  • Target synonyms
    CDA3, CDAIII, CDAN3, CDAN3A, CHO1, KNSL5, MKLP-1, MKLP1, kinesin-like protein KIF23, congenital dyserythropoietic anemia, type III, kinesin-like 5 (mitotic kinesin-like protein 1)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ02241
  • Protein Name
    Kinesin-like protein KIF23
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of KIF23 using anti-KIF23 antibody (M03483). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIF23 antigen affinity purified monoclonal antibody (Catalog # M03483) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KIF23 at approximately 98 kDa. The expected band size for KIF23 is at 98 kDa.
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