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ICC/IF analysis of Rar astrocytes cells using GTX47676 MLC1 antibody at 1:50. Left to right : MLC1, actin, merge.
ICC/IF analysis of Rar astrocytes cells using GTX47676 MLC1 antibody at 1:50. Left to right : MLC1, actin, merge.
ICC/IF analysis of Rar astrocytes cells using GTX47676 MLC1 antibody at 1:50. Left to right : MLC1, actin, merge.

MLC1 antibody, Internal

GTX47676
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetMLC1
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Overview

  • Supplier
    GeneTex
  • Product Name
    MLC1 antibody, Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.2-2.5 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID23209
  • Target name
    MLC1
  • Target description
    modulator of VRAC current 1
  • Target synonyms
    LVM; megalencephalic leukoencephalopathy with subcortical cysts 1; membrane protein MLC1; MLC; VL
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15049
  • Protein Name
    Membrane protein MLC1
  • Scientific Description
    The function of this gene product is unknown; however, homology to other proteins suggests that it may be an integral membrane transporter. Mutations in this gene have been associated with megalencephalic leukoencephalopathy with subcortical cysts, an autosomal recessive neurological disorder. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MLC1 using anti-MLC1 antibody (A02920-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat C6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MLC1 antigen affinity purified polyclonal antibody (Catalog # A02920-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MLC1 at approximately 41KD. The expected band size for MLC1 is at 41KD.
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