GeneTexs affinity purified anti-MLF1IP pT78 antibody was used at 20 microg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderately positive staining of mitotic cells in colon adenocarcinoma at 60X.? Tissue was formalin-fixed and paraffin embedded.? The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
GeneTexs affinity purified anti-MLF1IP pT78 antibody was used at 20 microg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderately positive staining of mitotic cells in colon adenocarcinoma at 60X.? Tissue was formalin-fixed and paraffin embedded.? The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
Mlf1 Interacting Protein (phospho Thr78) antibody
GTX48727
ApplicationsELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Human, Primate
TargetCENPU
Overview
- SupplierGeneTex
- Product NameMlf1 Interacting Protein (phospho Thr78) antibody
- Delivery Days Customer9
- ApplicationsELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.9 mg/ml
- ConjugateUnconjugated
- Gene ID79682
- Target nameCENPU
- Target descriptioncentromere protein U
- Target synonymsCENP50; CENPU50; centromere protein of 50 kDa; centromere protein U; interphase centromere complex protein 24; KLIP1; KSHV latent nuclear antigen interacting protein 1; MLF1 interacting protein; MLF1IP; PBIP1; polo-box-interacting protein 1
- HostRabbit
- IsotypeIgG
- Modification TypePhosphorylated
- Scientific DescriptionMyeloid leukemia factor-1 (MLF1) Interacting Protein (also known as PBIP1, MLF1IP1, KLIP1 or KSHV latent nuclear antigen interacting protein 1) is a novel polo-like kinase 1 (Plk1) substrate. Plk1 phosphorylation of MLF1IP induces ubiquitination and degradation of MLF1IP prior to the metaphase/ anaphase transition. Several Plk1-dependent phosphorylation sites have been identified on MLF1IP by mass spectrometry. Mutations of these sites stabilize MLF1IP and inhibit mitotic progression. Subsequent in vitro and in vivo MLF1IP phosphorylation and stability assays have revealed that phosphorylation of Thr78 is critical for triggering Plk1-dependent MLF1IP degradation. Expression of a non-degradable Thr78Ala mutant was sufficient to induce a mitotic block. Timely phosphorylation of MLF1IP on Thr78 by Plk1 is critical for eliminating the MLF1IP-imposed mitotic block prior to anaphase onset. MLF1IP is speculated to be a novel tumor suppressor.
- ReactivityBovine, Canine, Human, Primate
- Storage Instruction2°C to 8°C,-20°C or -80°C
- UNSPSC12352203