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Western Blot analysis of Jurkat cells using MLH1 Polyclonal Antibody
Western Blot analysis of Jurkat cells using MLH1 Polyclonal Antibody
Western Blot analysis of Jurkat cells using MLH1 Polyclonal Antibody

MLH1 Antibody

CSB-PA003247
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetMLH1
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Overview

  • Supplier
    Cusabio
  • Product Name
    MLH1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4292
  • Target name
    MLH1
  • Target description
    mutL homolog 1
  • Target synonyms
    COCA2; DNA mismatch repair protein Mlh1; FCC2; hMLH1; HNPCC; HNPCC2; MMRCS1; mutL homolog 1, colon cancer, nonpolyposis type 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP40692
  • Protein Name
    DNA mismatch repair protein Mlh1
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MLH1 using anti-MLH1 antibody (A00126-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human U251 whole cell lysates, Lane 6: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MLH1 antigen affinity purified polyclonal antibody (Catalog # A00126-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MLH1 at approximately 85 kDa. The expected band size for MLH1 is at 85 kDa.
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