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Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA014640LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA014640LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA014640LA01HU at dilution of 1:100

MLPH Antibody

CSB-PA014640LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetMLPH
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Overview

  • Supplier
    Cusabio
  • Product Name
    MLPH Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID79083
  • Target name
    MLPH
  • Target description
    melanophilin
  • Target synonyms
    SLAC2-A, melanophilin, exophilin-3, slp homolog lacking C2 domains a, synaptotagmin-like protein 2a, synaptotagmin-like protein homologue lacking C2 domains-a
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BV36
  • Protein Name
    Melanophilin
  • Scientific Description
    Rab effector protein involved in melanosome transport. Serves as link between melanosome-bound RAB27A and the motor protein MYO5A.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Melanophilin/MLPH using anti-Melanophilin/MLPH antibody (A06081-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Melanophilin/MLPH antigen affinity purified polyclonal antibody (Catalog # A06081-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Melanophilin/MLPH at approximately 85 kDa. The expected band size for Melanophilin/MLPH is at 66 kDa.
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