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Western Blot analysis of HuvEc cells using MMP-19 Polyclonal Antibody
Western Blot analysis of HuvEc cells using MMP-19 Polyclonal Antibody
Western Blot analysis of HuvEc cells using MMP-19 Polyclonal Antibody

MMP19 Antibody

CSB-PA003256
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetMMP19
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Overview

  • Supplier
    Cusabio
  • Product Name
    MMP19 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4327
  • Target name
    MMP19
  • Target description
    matrix metallopeptidase 19
  • Target synonyms
    CODA; matrix metalloproteinase RASI; matrix metalloproteinase-18; matrix metalloproteinase-19; matrix metalloproteinase-beta19; MMP18; RASI-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99542
  • Protein Name
    Matrix metalloproteinase-19
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MMP19 using anti-MMP19 antibody (A05171-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP19 antigen affinity purified polyclonal antibody (Catalog # A05171-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP19 at approximately 54 kDa. The expected band size for MMP19 is at 57 kDa.
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