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MMP9 antibody

MMP9 antibody

GTX27299
GeneTex
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityRat
TargetMmp9
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Overview

  • Supplier
    GeneTex
  • Product Name
    MMP9 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    For IP: Use at a dilution of 1:500. For WB: Use at a dilution of 1:2000. Optimal dilutions/concentrations should be determined by the researcher.
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID81687
  • Target name
    Mmp9
  • Target description
    matrix metallopeptidase 9
  • Target synonyms
    matrix metalloproteinase-9, 92 kDa gelatinase, 92-kDa type IV collagenase, GELB, MMP-9, gelatinase B, matrix metalloproteinase 9 (gelatinase B, 92-kDa type IV collagenase)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP50282
  • Protein Name
    Matrix metalloproteinase-9
  • Scientific Description
    metalloproteinase involved in extracellular matrix remodeling and bone resorption [RGD, Feb 2006]
  • Reactivity
    Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MMP-9 using anti-MMP-9 antibody (PB10008). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: NRK whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP-9 antigen affinity purified polyclonal antibody (Catalog # PB10008) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP-9 at approximately 78 kDa, 92 kDa. The expected band size for MMP-9 is at 79 kDa.
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