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WB analysis of K562 cell lysates using GTX87017 MMTAG2 antibody. The lane on the right is blocked with the synthesized peptide.
WB analysis of K562 cell lysates using GTX87017 MMTAG2 antibody. The lane on the right is blocked with the synthesized peptide.
WB analysis of K562 cell lysates using GTX87017 MMTAG2 antibody. The lane on the right is blocked with the synthesized peptide.

MMTAG2 antibody

GTX87017
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetC1orf35
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Overview

  • Supplier
    GeneTex
  • Product Name
    MMTAG2 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500~1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID79169
  • Target name
    C1orf35
  • Target description
    chromosome 1 open reading frame 35
  • Target synonyms
    MMTAG2, multiple myeloma tumor-associated protein 2, multiple myeloma transforming 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BU76
  • Protein Name
    Multiple myeloma tumor-associated protein 2
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of C1orf35/MMTAG2 using anti-C1orf35/MMTAG2 antibody (A14711-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C1orf35/MMTAG2 antigen affinity purified polyclonal antibody (Catalog # A14711-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C1orf35/MMTAG2 at approximately 35 kDa. The expected band size for C1orf35/MMTAG2 is at 29 kDa.
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