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Western Blot analysis of 293 cells using Mat1 Polyclonal Antibody
Western Blot analysis of 293 cells using Mat1 Polyclonal Antibody
Western Blot analysis of 293 cells using Mat1 Polyclonal Antibody

MNAT1 Antibody

CSB-PA003197
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMNAT1
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Overview

  • Supplier
    Cusabio
  • Product Name
    MNAT1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4331
  • Target name
    MNAT1
  • Target description
    MNAT1 component of CDK activating kinase
  • Target synonyms
    CAP35, MAT1, RNF66, TFB3, CDK-activating kinase assembly factor MAT1, CDK7/cyclin-H assembly factor, MNAT CDK-activating kinase assembly factor 1, MNAT1, CDK activating kinase assembly factor, RING finger protein 66, RING finger protein MAT1, cyclin G1 interacting protein, menage a trois 1 (CAK assembly factor), menage a trois homolog 1, cyclin H assembly factor, menage a trois-like protein 1 cyclin H assembly factor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51948
  • Protein Name
    CDK-activating kinase assembly factor MAT1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of MNAT1 using anti-MNAT1 antibody (A06523). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A375 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MNAT1 antigen affinity purified polyclonal antibody (Catalog # A06523) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MNAT1 at approximately 35 kDa. The expected band size for MNAT1 is at 35 kDa.
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