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Western blot All lanes: MOCS1 antibody at 5.09 ug/ml + Mouse heart tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 71, 44, 41, 32, 69, 59 kDa Observed band size: 71 kDa
Western blot All lanes: MOCS1 antibody at 5.09 ug/ml + Mouse heart tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 71, 44, 41, 32, 69, 59 kDa Observed band size: 71 kDa
Western blot All lanes: MOCS1 antibody at 5.09 ug/ml + Mouse heart tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 71, 44, 41, 32, 69, 59 kDa Observed band size: 71 kDa

MOCS1 Antibody

CSB-PA878934ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetMOCS1
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Overview

  • Supplier
    Cusabio
  • Product Name
    MOCS1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4337
  • Target name
    MOCS1
  • Target description
    molybdenum cofactor synthesis 1
  • Target synonyms
    cell migration-inducing gene 11 protein; MIG11; MOCOD; MOCS1A; MOCS1A enzyme; MOCS1B; molybdenum cofactor biosynthesis protein 1; molybdenum cofactor biosynthesis protein A; molybdenum cofactor synthesis-step 1 protein A-B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NZB8
  • Protein Name
    Molybdenum cofactor biosynthesis protein 1
  • Scientific Description
    Isoform MOCS1A and isoform MOCS1B probably form a complex that catalyzes the conversion of 5-GTP to cyclic pyranopterin monophosphate (cPMP or molybdopterin precursor Z).
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MOCS1 using anti-MOCS1 antibody (A07628-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human U20S whole cell lysates, Lane 6: human 293T whole cell lysates, Lane 7: human SH-SY5Y whole cell lysates, Lane 8: human RT4 whole cell lysates, Lane 9: rat liver tissue lysates, Lane 10: rat PC-12 whole cell lysates, Lane 11: mouse liver tissue lysates, Lane 12: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MOCS1 antigen affinity purified polyclonal antibody (Catalog # A07628-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MOCS1 at approximately 58 kDa. The expected band size for MOCS1 is at 70 kDa.
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