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Western blot All lanes: MOCS2 antibody at 1.68 ug/ml Lane 1: A549 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 21 kDa Observed band size: 21 kDa
Western blot All lanes: MOCS2 antibody at 1.68 ug/ml Lane 1: A549 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 21 kDa Observed band size: 21 kDa
Western blot All lanes: MOCS2 antibody at 1.68 ug/ml Lane 1: A549 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 21 kDa Observed band size: 21 kDa

MOCS2 Antibody

CSB-PA014706ESR1HU
Cusabio
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMOCS2
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Overview

  • Supplier
    Cusabio
  • Product Name
    MOCS2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4338
  • Target name
    MOCS2
  • Target description
    molybdenum cofactor synthesis 2
  • Target synonyms
    MCBPE, MOCO1, MOCODB, MPTS, molybdopterin synthase catalytic subunit, molybdopterin synthase sulfur carrier subunit, molybdenum cofactor biosynthesis protein E
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO96007
  • Protein Name
    Molybdopterin synthase catalytic subunit
  • Scientific Description
    Acts as a sulfur carrier required for molybdopterin biosynthesis. Component of the molybdopterin synthase complex that catalyzes the conversion of precursor Z into molybdopterin by mediating the incorporation of 2 sulfur atoms into precursor Z to generate a dithiolene group. In the complex, serves as sulfur donor by being thiocarboxylated (-COSH) at its C-terminus by MOCS3. After interaction with MOCS2B, the sulfur is then transferred to precursor Z to form molybdopterin.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MOCS2 using anti-MOCS2 antibody (A07401). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human LNCAP whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: human Jurkat whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human HepG2 whole cell lysates, Lane 8: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MOCS2 antigen affinity purified polyclonal antibody (Catalog # A07401) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MOCS2 at approximately 21 kDa. The expected band size for MOCS2 is at 21 kDa.
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