MOCS3 Antibody

Cusabio

MOCS3 Antibody

20

Western Blot, ELISA, ImmunoHistoChemistry

Research Use Only

Polyclonal

Unconjugated

MOCS3

molybdenum cofactor synthesis 3

adenylyltransferase and sulfurtransferase MOCS3; molybdenum cofactor synthesis protein 3; molybdopterin synthase sulfurylase; MPT synthase sulfurylase; UBA4; UBA4, ubiquitin-activating enzyme E1 homolog; ubiquitin-like modifier activating enzyme 4

Rabbit

IgG

Adenylyltransferase and sulfurtransferase MOCS3

Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions.

Human

-20°C or -80°C

41116161