ELISA Results of Rabbit Anti-Monkey IgG Antibody Alkaline Phosphatase Conjugate tested against purified Monkey IgG Alk Phos. Each well was coated in duplicate with 1.0 microg of Monkey IgG (p/n 017-0102). The working dilution is 1:4300. The starting dilution of antibody was 5microg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Substrate (p/n MB-076), ELISA Alkaline Phosphatase Substrate Buffer (p/n NPP-10) and NPP Working Buffer (p/n NPP-B500).