Mouse Ang1-7(Angiotensin1-7) ELISA Kit

Biorbyt

Mouse Ang1-7(Angiotensin1-7) ELISA Kit

10

standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Ang1-7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Ang1-7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Ang1-7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mouse Ang1-7 in the samples is then determined by comparing the OD of the samples to the standard curve

31.25-2000 pg/mL

19.07 pg/mL

Research Use Only

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiotensin1-7(Ang1-7). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Angiotensin1-7(Ang1-7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiotensin1-7(Ang1-7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Angiotensin1-7(Ang1-7) in the samples is then determined by comparing the OD of the samples to the standard curve.

Mouse

41116158