Mouse anti actin gamma-cytoplasmic
MUB0111P-CE/IVD
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityChicken, Fish, Human, Mouse, Rabbit, Rat
Overview
- SupplierNordic-MUbio
- Product NameMouse anti actin gamma-cytoplasmic
- Delivery Days Customer7
- ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
- Applications SupplierImmunohistochemistry (frozen);Immunohistochemistry (paraffin);Western Blotting;Flow Cytometry;ELISA
- Category SupplierPrimary antibodies
- CertificationResearch Use Only
- Clone ID2A3
- HostMouse
- IsotypeIgG2b
- Source2A3 is a Mouse monoclonal IgG2b antibody derived by fusion of NS0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a peptide comprising the N-terminal nonapeptide of gamma-cytoplasmic actin with an acetylated N-terminus (Ac-EEEIAAL
- ReactivityChicken, Fish, Human, Mouse, Rabbit, Rat
- Reactivity SupplierChicken;Human;Mouse;Rabbit;Rat;Swine;Zebrafish
- Storage InstructionThe antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
- UNSPSC12352203
References
- 1. Vandekerckhove, J. and Weber, K. (1978). At least six different actins are expressed in a higher mammal: an analysis based on the amino acid sequence of the amino-terminal tryptic peptide. J Mol Biol. 126(4), 783-802. 2. Bassell, G.J., Zhang, H., Byrd, A.L., Femino, A.M., Singer, R.H., Taneja, K.L., Lifshitz, L.M., Herman, I.M. and Kosik, K.S. (1998). Sorting of beta-actin mRNA and protein to neurites and growth cones in culture. J Neurosci. 1998 18(1), 251-65. 3. Dugina, V., Zwaenepoel, I., Gabbiani, G., Clément, S. and Chaponnier, C. (2009). ß- and y-cytoplasmic actins display distinct distribution and functional diversity. J Cell Sci 122, 2980-88. 4. Gallant, C., Appel, S., Graceffa, P., Leavis, P., Lin, J.J., Gunning, P.W., Schevzov, G., Chaponnier, C., Degnore, J., Lehman, W. and Morgan, K.G. (2011). Tropomyosin variants describe distinct functional subcellular domains in differentiated vascular smooth muscle cells. Am J Physiol Cell Physiol. 300(6), 1356-65.