Mouse anti actin gamma-cytoplasmic

Nordic-MUbio

Mouse anti actin gamma-cytoplasmic

7

2A3 is a mouse monoclonal IgG2b antibody highly specific for gamma-cytoplasmic actin (gamma-actin 1; encoded by ACTG1). It does not cross-react with the gamma-actin isoform expressed in the enteric smooth muscle tissues (gamma-actin 2; encoded by ACTG2).

2A3 is useful for immunocytochemistry on methanol fixed cells, immunohistochemistry on methanol fixed frozen sections and paraffin-embedded tissues, immunoblotting, flow cytometry and ELISA. Optimal antibody dilution should be determined by titration; recommended range is 1:100 - 1:500 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:1000 - 1:5000 for immunoblotting applications.

Immunohistochemistry (frozen);Immunohistochemistry (paraffin);Western Blotting;Flow Cytometry;ELISA

Primary antibodies

2A3

ACTG1

actin gamma 1

ACT; ACTG; actin, cytoplasmic 2; cytoskeletal gamma-actin; DFNA20; DFNA26; epididymis luminal protein 176; HEL-176

Actin, cytoplasmic 2

2A3 is a Mouse monoclonal IgG2b antibody derived by fusion of NS0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a peptide comprising the N-terminal nonapeptide of gamma-cytoplasmic actin with an acetylated N-terminus ...

Chicken;Human;Mouse;Rabbit;Rat;Swine;Zebrafish

12352203