Mouse anti actin gamma-cytoplasmic
MUB0111P-CE/IVD
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityChicken, Human, Mouse, Porcine, Rabbit, Rat, Zebra Fish
TargetACTG1
Overview
- SupplierNordic-MUbio
- Product NameMouse anti actin gamma-cytoplasmic
- Delivery Days Customer7
- Antibody Specificity2A3 is a mouse monoclonal IgG2b antibody highly specific for gamma-cytoplasmic actin (gamma-actin 1; encoded by ACTG1). It does not cross-react with the gamma-actin isoform expressed in the enteric smooth muscle tissues (gamma-actin 2; encoded by ACTG2).
- Application Supplier Note2A3 is useful for immunocytochemistry on methanol fixed cells, immunohistochemistry on methanol fixed frozen sections and paraffin-embedded tissues, immunoblotting, flow cytometry and ELISA. Optimal antibody dilution should be determined by titration; recommended range is 1:100 - 1:500 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:1000 - 1:5000 for immunoblotting applications.
- ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierImmunohistochemistry (frozen);Immunohistochemistry (paraffin);Western Blotting;Flow Cytometry;ELISA
- Category SupplierPrimary antibodies
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID2A3
- Gene ID71
- Target nameACTG1
- Target descriptionactin gamma 1
- Target synonymsACT; ACTG; actin, cytoplasmic 2; cytoskeletal gamma-actin; DFNA20; DFNA26; epididymis luminal protein 176; HEL-176
- HostMouse
- IsotypeIgG2b
- Protein IDP63261
- Protein NameActin, cytoplasmic 2
- Source2A3 is a Mouse monoclonal IgG2b antibody derived by fusion of NS0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a peptide comprising the N-terminal nonapeptide of gamma-cytoplasmic actin with an acetylated N-terminus ...
- ReactivityChicken, Human, Mouse, Porcine, Rabbit, Rat, Zebra Fish
- Reactivity SupplierChicken;Human;Mouse;Rabbit;Rat;Swine;Zebrafish
- UNSPSC12352203
References
- 1. Vandekerckhove, J. and Weber, K. (1978). At least six different actins are expressed in a higher mammal: an analysis based on the amino acid sequence of the amino-terminal tryptic peptide. J Mol Biol. 126(4), 783-802. 2. Bassell, G.J., Zhang, H., Byrd, A.L., Femino, A.M., Singer, R.H., Taneja, K.L., Lifshitz, L.M., Herman, I.M. and Kosik, K.S. (1998). Sorting of beta-actin mRNA and protein to neurites and growth cones in culture. J Neurosci. 1998 18(1), 251-65. 3. Dugina, V., Zwaenepoel, I., Gabbiani, G., Clément, S. and Chaponnier, C. (2009). ß- and y-cytoplasmic actins display distinct distribution and functional diversity. J Cell Sci 122, 2980-88. 4. Gallant, C., Appel, S., Graceffa, P., Leavis, P., Lin, J.J., Gunning, P.W., Schevzov, G., Chaponnier, C., Degnore, J., Lehman, W. and Morgan, K.G. (2011). Tropomyosin variants describe distinct functional subcellular domains in differentiated vascular smooth muscle cells. Am J Physiol Cell Physiol. 300(6), 1356-65.