Mouse anti annexin V
MUB0106P
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Zebra Fish
TargetANXA5
Overview
- SupplierNordic-MUbio
- Product NameMouse anti annexin V
- Delivery Days Customer7
- Antibody SpecificityRUU-WAC2A reacts anti Human recombinant and enDogenous annexin A5. It shows no cross reactivity with Annexins I, II, III, IV, VI, VII and VIII in ELISA or in immunoprecipitation. It does not recognise Monkey and Rat Annexin V in ELISA.
- Application Supplier NoteThe monoclonal antibody is suitable for ELISA in combination with a polyclonal antibody raised anti Annexin V in Rabbit. It can be applied in immunofluorescence to parafolmaldehyde fixed cell samples and in immunohistochemistry of frozen sections and formaldehyde fixed and paraffin embedded tissue specimens. For immunohistochemistry of paraffin sections a pretreatment step with 2% amino-propyl-triethoxysilan (in 100% ethanol) and 0,2 % BSA is recommended. The sections are heated for 15 minutes at 100°C in a citRate buffer (10 mM trisodium citRate, pH 6,0). The sections are then further processed by routine procedures (see below). RUU-WAC2a inhibits annexin A5 binding to anionic phospholipid-containing vesicles, activated platelets and apoptotic cells for more than 95%. Optimal antibody dilution should be determined by titration; recommended range is 1:50 - 1:100 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:500 for immunoblotting applications.
- ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierELISA;Immunocytochemistry;Immunofluorescence;Immunohistochemistry (frozen);Immunohistochemistry (paraffin)
- Category SupplierPrimary antibodies
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDRUU-WAC2A
- Gene ID308
- Target nameANXA5
- Target descriptionannexin A5
- Target synonymsanchorin CII; annexin A5; annexin V; annexin-5; ANX5; calphobindin I; CBP-I; endonexin II; ENX2; epididymis secretory protein Li 7; HEL-S-7; lipocortin V; PAP-I; placental anticoagulant protein 4; placental anticoagulant protein I; PP4; RPRGL3; thromboplastin inhibitor; VAC-alpha; vascular anticoagulant-alpha
- HostMouse
- IsotypeIgG1
- Protein IDP08758
- Protein NameAnnexin A5
- SourceRUU-WAC2a is a Mouse monoclonal IgG1 antibody obtained by fusion of P3-X63-Ag 8,653 Mouse myeloma cells with spleen cells from a BABL/c Mouse immunized with recombinant annexin A5.
- ReactivityHuman, Zebra Fish
- Reactivity SupplierHuman;Zebrafish
- UNSPSC12352203
References
- 1. Koopman, G., Reutelingsperger, C.P., Kuijten, G.A.M. et al. (1994). Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis. Blood 84, 1415-20. 2. Vermes, I., Haanen, C., Steffens-Nakken, H. and Reutelingsperger, C. (1995). A novel assay for apoptosis-flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V. J Immunol Methods 184, 39. 3. Wen, Y., Edelman J.L., Kang, T. and Sachs, G. (1999). Lipocortin V may function as a signaling protein for vascular endothelial growth factor receptor-2/Flk-1. Biochem. Biophys. Res. Commun. 258, 713–21. 4. Cardó-Vila, M., Arap, W. and Pasqualini, R. (2003). Alpha v beta 5 integrin-dependent programmed cell death triggered by a peptide mimic of annex V. Mol. Cell 31, 1151-62. 5. Van Heerde, W.L. (1994). Thesis; localization, plasma levels and anticoagulant activity. 6. Van Heerde, W.L., Reutelingsperger, C.P., Maassen, C., Lux, P., Derksen, R.H. and De Groot, P.G. (2003). The presence of antiphospholipid antibodies is not related to increased levels of annexin A5 in plasma. Jn Thromb Haemost. 3, 532-536. 7. Van Heerde, W.L., Lap, P., Schoormans, S., De Groot, P.G., Reutelingsperger, C.P.M. and Vroom, T.M. (2004). Localization of annexin A5 in Human tissues. Annexins 1, 37-43.