Mouse anti annexin V

Nordic-MUbio

Mouse anti annexin V

7

The monoclonal antibody is suitable for ELISA in combination with a polyclonal antibody raised anti Annexin V in Rabbit. It can be applied in immunofluorescence to parafolmaldehyde fixed cell samples and in immunohistochemistry of frozen sections and formaldehyde fixed and paraffin embedded tissue specimens. For immunohistochemistry of paraffin sections a pretreatment step with 2% amino-propyl-triethoxysilan (in 100% ethanol) and 0,2 % BSA is recommended. The sections are heated for 15 minutes at 100°C in a citRate buffer (10 mM trisodium citRate, pH 6,0). The sections are then further processed by routine procedures (see below). RUU-WAC2a inhibits annexin A5 binding to anionic phospholipid-containing vesicles, activated platelets and apoptotic cells for more than 95%. Optimal antibody dilution should be determined by titration; recommended range is 1:50 - 1:100 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:500 for immunoblotting applications.

ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

ELISA;Immunocytochemistry;Immunofluorescence;Immunohistochemistry (frozen);Immunohistochemistry (paraffin)

Primary antibodies

Research Use Only

Monoclonal

RUU-WAC2A

ANXA5

annexin A5

anchorin CII; annexin A5; annexin V; annexin-5; ANX5; calphobindin I; CBP-I; endonexin II; ENX2; epididymis secretory protein Li 7; HEL-S-7; lipocortin V; PAP-I; placental anticoagulant protein 4; placental anticoagulant protein I; PP4; RPRGL3; thromboplastin inhibitor; VAC-alpha; vascular anticoagulant-alpha

Mouse

IgG1

Annexin A5

RUU-WAC2a is a Mouse monoclonal IgG1 antibody obtained by fusion of P3-X63-Ag 8,653 Mouse myeloma cells with spleen cells from a BABL/c Mouse immunized with recombinant annexin A5.

Human;Zebrafish

12352203