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ProductsBack/Mouse anti Carcinoembryonic Antigen / CEA

Mouse anti Carcinoembryonic Antigen / CEA

MUB0332P
Nordic-MUbio
ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCEACAM5
0.1 mg
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Mouse anti Carcinoembryonic Antigen / CEA
  • Delivery Days Customer
    7
  • Application Supplier Note
    PARLAM 4 is useful for flow cytometry, immunoblotting, immunocytochemistry on methanol fixed cells and immunohistochemistry on frozen tissues when using a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2. The antibody is also reactive in formalin-fixed and paraffin-embedded tissue sections after treatment with citrate buffer pH 6.0 in an autoclave. Human colon carcinoma tissue is used as positive control. Optimal antibody dilution should be determined by titration; we recommend a 1:25 - 1:100 dilution for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:500 for immunoblotting application.
  • Applications
    Flow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    Flow Cytometry;Immunocytochemistry;Immunohistochemistry (frozen);Immunohistochemistry (paraffin);Western Blotting
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    PARLAM 4
  • Gene ID1048
  • Target name
    CEACAM5
  • Target description
    CEA cell adhesion molecule 5
  • Target synonyms
    carcinoembryonic antigen related cell adhesion molecule 5; carcinoembryonic antigen-related cell adhesion molecule 5; CD66e; CEA; meconium antigen 100
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP06731
  • Protein Name
    Carcinoembryonic antigen-related cell adhesion molecule 5
  • Source
    PARLAM 4 is a mouse monoclonal IgG1 antibody derived by fusion of Sp 2/0 Ag 14 Mouse myeloma cells with spleen cells from a BABL/c Mouse immunized with isolated Human CEA. The immunogen has been isolated from Human colonic carcinoma cells.
  • Reactivity Supplier
    Human
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CEA/CEACAM5 using anti-CEA/CEACAM5 antibody (A00356-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEA/CEACAM5 antigen affinity purified polyclonal antibody (Catalog # A00356-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEA/CEACAM5 at approximately 200 kDa. The expected band size for CEA/CEACAM5 is at 77,180-200 kDa.
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